The development and use of bioplastic is one of the emerging methods to tackle the issue of ever-increasing plastic waste1. Lip1 is an intracellular lipase from Pseudomonas chlororaphis PA23 capable of degrading several types of bioplastics, including polyhydroxyalkanoates (PHA), polycaprolactone (PCL), polylactic acid (PLA), and polyethylene succinate (PES)3. Using a sequence similarity network (SSN) of homologs of PcLip1, a hypothetical protein present in Aliidongia dinghuensis named AdLip1 has been found to have increased thermostability. We have explored the evolution of this family of broad-spectrum bioplastic degrading enzymes using Ancestral sequence reconstruction, which has allowed us to discover synthetic ancestral sequences and compare them structurally against the crystal structure of PcLip1.